Isozymes: Methods and Applications

P-178: Separation and Identification of Alkaline Phosphatase Isozymes during Pregnancy

Background: Alkaline phosphatase (ALP), (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from various molecules in the body. In humans, ALPis present in all tissues such as liver, bile duct, kidney, bone, and the placenta which detection of its activity is so useful in molecular biology. Pregnancy is associated with normal physiological changes that assist fetal surv...

Antioxidant Isozymes Activities in Potato Plants (Solanum tuberosum L.) Under Salt Stress

Peroxide isozymes and Malondialdehyde content and ascorbate peroxidase activity in Berberis integerrima and Cercis siliquastrum under Cd-induced stress .

ROS detoxification is an essential process in protecting plant cells and their organelles, which is caused by ROS generated in stress condition. Peroxidase (POD) isozyme, Malondialdehyde (MDA) content, and Ascorbate peroxidase (APX) activity are the indicators of plants for measuring pollution effects. The purpose of the present study was to investigate Cd-induced stress on POD isozymes, MDA co...

Specific determination of N-acetyl-beta-D-hexosaminidase isozymes A and B by radioimmunoassay and radial immunodiffusion.

The two major isozymes of N-acetylhexosaminidase, namely hexosaminidases A and B were quantitatively determined in tissues and biological fluids of both normal individuals and Tay-Sachs patients. The determination was carried out by two sensitive immunoassays:radial immunodiffusion, using chromogenic substrate, and radioimmunoassay, which were developed in this study. For this purpose [correcte...

Analysis of Tissue Specific Expression Patterns of Isozymes

Isozymes are enzymes catalyzing the same biochemical reaction, but differ in amino acid sequences. They usually display different kinetic parameters, or express different regulatory properties. In addition some isozymes show tissue-specific expressions. These differences are considered that isozymes have properties related to the tissue-specific environments. Aldolase, one example of isozymes, ...